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KMID : 0605619980050020219
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Journal of Korean Society of Biological Psychiatry
1998 Volume.5 No. 2 p.219 ~ p.226
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Cytosine Arabinoside-Induced PC12 Cell Death Pathway
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1양보기/1Bo Gee Yang
1채영규/2양병환/1Young Gyu Chai/2Byung Hwan Yang
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Abstract
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Cytosine arabinoside(AraC) inhibits DNA synthesis and β-DNA polymerase, an
enzyme involved in DNA repair. This a potent antimitotic agent, is clinically used as an
anticancer drug with side effect of severe neurotoxicity. Earlier reports suggested that
inhibition of neuronal survival by AraC in sympathetic neuron may be due to the
inhibition of a 2'-deoxycytidine-dependent process that is independent of DNA synthesis
or repair and AraC induced a signal that is triggers a cascade of new mRNA and
protein synthesis, leading to apoptotic cell death in cultured cerebellar granule cells. The
present study would suggest whether caspase family(ICE/CED-3-like protease) involved
in AraC-induced apoptosis pathway of PC12 cells. It was observed that treatment of
PC12 cells with AraC led to decrease of viability by MTT assay and morphology
changes, which did not suggest that AraC induced apoptosis in PC12 cells. The mRNA
of caspase-1/caspase-3 were expressed in PC12 cells constitutively, and AraC did not
activate caspase family. These results suggest that caspase-1/caspase-3 may not be
required for AraC-induced cell death pathway in PC12 cells.
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